The Brazilian Journal of Infectious Diseases The Brazilian Journal of Infectious Diseases
Braz J Infect Dis 2017;21:290-6 - Vol. 21 Num.3 DOI: 10.1016/j.bjid.2017.02.002
Original article
Bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the Caatinga biome, Northeastern Brazil: a serological and molecular study
Mariana Campos Fontalvoa, Alexsandra Rodrigues de Mendonça Favachob, Andreina de Carvalho Araujoa, Naylla Mayana dos Santosa, Glauber Meneses Barboza de Oliveiraa, Daniel Moura Aguiarc, Elba Regina Sampaio de Lemosb, Mauricio Claudio Hortaa,,
a Universidade Federal do Vale do São Francisco (Univasf), Laboratório de Doenças Parasitárias, Petrolina, PE, Brazil
b Instituto Oswaldo Cruz (FIOCRUZ), Laboratório de Hantaviroses e Rickettsioses, Rio de Janeiro, RJ, Brazil
c Universidade Federal de Mato Grosso, Laboratório de Virologia e Rickettsioses, Cuiabá, MT, Brazil
Received 04 October 2016, Accepted 03 February 2017
Abstract

This study verified the occurrence of Bartonella spp. in dogs, cats, wild mammals and their ectoparasites in Petrolina and Lagoa Grande Counties, Pernambuco, located in a semi-arid region in Northeastern Brazil. Anti-Bartonella spp. antibodies were detected by indirect immunofluorescence assay (IFA) in 24.8% of dogs (27/109) and in 15% of cats (6/40). Bartonella sp. DNA was identified by PCR performed on DNA extracted from blood and ectoparasites using primers targeting Bartonella sp. gltA and ribC genes in 100% (9/9) of Pulex irritans from Cerdocyon thous, 57.4% (35/61) of P. irritans from dogs, 2.3% (1/43) of Ctenocephalides felis felis from dogs, 53.3% (24/45) of C. felis felis from cats, and 10% (1/10) of Polyplax spp. from Thrichomys apereoides. DNA sequencing identified Bartonella clarridgeiae and Bartonella henselae in C. felis felis from cats, Bartonella rochalimae in P. irritans from dog and C. thous, and Bartonella vinsoni berkhofii in P. irritans from dog.

Keywords
Bartonella, Flea, Tick, Lice, Brazil
Braz J Infect Dis 2017;21:290-6 - Vol. 21 Num.3 DOI: 10.1016/j.bjid.2017.02.002