The Brazilian Journal of Infectious Diseases The Brazilian Journal of Infectious Diseases
Braz J Infect Dis 2017;21:248-54 - Vol. 21 Num.3 DOI: 10.1016/j.bjid.2017.03.002
Original article
Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression
Ana Carolina Silva Chuerya,, , Ismael Dale Cotrim Guerreiro da Silvab, Julisa Chamorro Lascasas Ribaltaa, Neila Maria de Góis Specka
a Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Núcleo de Prevenção de Doenças Ginecológicas, São Paulo, SP, Brazil
b Universidade Federal de São Paulo (Unifesp), Departamento de Ginecologia, Escola Paulista de Medicina, São Paulo, SP, Brazil
Received 03 August 2016, Accepted 02 March 2017
Abstract
Objective

To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression.

Methods

We analyzed 175 cervical samples from women aged 16–69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n=75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n=52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n=48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results

The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p=0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p<0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633; 95% CI, 1.399–4.954, p=0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups.

Conclusion

The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression.

Keywords
Human papillomavirus, Oncoproteins, Genetic polymorphisms, p53 gene, Arginine
Braz J Infect Dis 2017;21:248-54 - Vol. 21 Num.3 DOI: 10.1016/j.bjid.2017.03.002